Hints & Tips

Welcome to Our Hints and Tips Section, Please choose a link from below.

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When To ReValidate Your Methods

Gas Chromatography Tips

HPLC Tips

Syringe Tips

 

  

Gas Chromatography Tips

  • When installing your column into an FID jet, never pass the column through the flame. This will burn the inner (phase) and outer (polyimide) coatings and will cause higher background signals.
  • Purge column with oxygen-free carrier gas for at least 30 minutes before heating GC oven.
  • When storing a capillary column, seal the column ends with a used septum. Remember to cut at least 3cm from each column end before re-installation.
  • If you're having problems with solvent focusing, or early eluting peaks seem broad or lop-sided in splitless injection, then try using a column with a thicker film.
  • Set the FID temperature 20°C above the maximum method temperature, 250°C is usually the recommended temperature.
  • When peak shape deteriorates, replace the liner immediately and cut 30cm from the front end of the column.
  • When changing your column, liner or septum, SGE recommends lowering the injector temperature to <100°C to prevent stripping the liner deactivation. This is especially important for the analysis of trace levels of active compounds.
  • Always re-tighten Graphite / Vespel® ferrules by ¼ to ½ turn after the first 2-3 oven cycles.
  • To prevent increasing retention times in your chromatography, replace the septum daily.
  • Condition column at: a. 20°C above the method temperature or
    b. the recommended maximum column temperature. (whichever temperature is lower)
  • If the injection port temperature is not specified in the method, 250°C is usually the recommended temperature.
  • Always use SilTiteT or Graphite/Vespel® ferrules when connecting a column to a GC/MS interface.
  • For maximum FID sensitivity, set the air flow 10 times higher than the hydrogen flow.
  • To extend the lifetime of your packed column, ensure the oven temperature is 50°C, or less, before turning off the carrier gas.
  • Do not use plastic tubing in GC systems. Plastic tubing, when used for general plumbing, can absorb up to 20% moisture allowing external laboratory gases to transpire through the tubing. SGE recommends stainless steel tubing to be used throughout the GC system.
  • When initialising a new Column, always condition with the detector end removed from the detector port.  This prevents stationary phase coating detector internals leading to high noise and reduced sensitivity.

HPLC Tips

  • SGE HPLC fittings, guard cartridges and in-line filters are completely compatible with all other manufacturers' HPLC columns and systems.
  • If you are doing any method development with reversed phase HPLC, Wakosil II 5C18 RS columns should be the first columns to try.
  • Always use a guard cartridge to prolong column life.
  • For quick and easy column connections use EasyLOK nuts.
  • If your reversed phase HPLC run is taking too long on a C18 or ODS column try using a SGE Wakosil II 5C8 RS (basic compounds and method development), Exsil C8 (existing method on an older style ODS), or Nucleosil C8 (protein analysis and peptides).
  • SGE Guard cartridges can be used in conjunction with competitors' columns (for those customers with methods validated on other columns).
  • PEEKsil tubing is an excellent alternative to PEEK tubing as its internal bore is unaffected by any organic solvents.
  • Wakosil II 5C18 AR columns have a pH range of 1.4 - 9.4 making it a very TOUGH phase.
  • If you are close to the limit of detection using a standard 4.6mm or 4mm ID HPLC column try using a 2mm ID column. This saves solvent too.
  • For complete loop fill, the syringe capacity should be greater than twice the loop volume. The loop capacity sets the injection volume. For partial loop fill, the injection volume should be no greater than half the loop capacity. The injection size sets the injection volume.
  • If you can't pre-filter samples, make sure to use a low dead volume in-line filter after the injector.
  • Always ensure buffers are fully washed from your system using water (warm if possible).  If a sample sequence completes during the night, change pump flow to 0.1ml/min rather than stopping completely.

Syringe Tips

  • To maximize accuracy and reproducibility, it is recommended that the minimum volume injected from a syringe is 20% of the total syringe capacity.
  • To eliminate carryover between samples, flush the syringe with solvent 15-20 times, remembering to discard the first 2-3 washes.
  • Achieve a 1% RSD instantly by presetting the syringe volume.
  • Large volume injection syringes are ideal for temperature programmable split injection.
  • For best syringe life, ensure your syringe is rinsed 5 to 10 times in clean solvent prior to storage.

 

 

 

 

 

 

Welcome to Antech Solutions Ltd Website

Antech are the premier Chromatography & Tablet Dissolution, Disintegration Qualification, Sales, Service and Training company in Ireland. We supply Chromatography Consumables and Instrumentation.  We specialise in Handheld Spectroscopy and are the sole distributor in Ireland and the UK for Thermo Fisher Scientifics PAI (Portable Analytical Instruments Division) for Handheld Spectrometers including: TruScan - Innovative Raman Handheld Spectrometer, TruDefender - Handheld FTIR Spectrometer,  microPHAZIR (polychromix) - Portable Handheld NIR Spectrometer.  Niton XRF - Handheld X-Ray.  Portable GMP - Handheld GMP. Chromatography Supplies. Dissolution and Disintegration Supplies. Portable Analyser, Handheld Analyzer, Portable NIR Analyzer, Handheld NIR Analyser. Handheld (Portable) Raman, NIR and FTIR Analysers (Analyzers).

Our complete laboratory services include Validation, User Training courses plus Service Contracts and Maintenance for a complete range of HPLC, GC and Tablet Dissolution instrumentation. HTA Headspace and Liquid GC autosamplers are available now to operate with your existing GC including:


TruScan handheld Raman system for rapid raw material verification. Portable GMP; Handheld GMP. Non-destructive point-and-shoot sampling through sealed packaging delivers results in just seconds. Thermo Scientific - Portable Optical Analyis - TruScan Raman handheld spectrometer reduces the need for quarantine areas and staging of raw materials and minimizes risk of exposure and contamination. Compliant to all relevant global regulatory, safety and commercial requirements, including UL intrinsic safety certification and support for 21 CFR Part 11. Portable cGMP - Handheld cGMP. Thermo Scientific (Portable Optical Analysis Division) TruScan (Raman Handheld/Portable Spectrometer) for Anti-Counterfeit. Raman Spectroscopy. Handheld Spectroscopy. New TruScan RM - Faster, Lighter Handheld Portable Raman Analyser, Spectrometer.  TruDefenderFT, Handheld FTIR Spectometer, Portable FTIR, Analytical FTIR.  Portable NIR, Handheld NIR, microPHAZIR Handheld NIR Analyser, Portable NIR spectrometer, Handheld NIR spectrometer.  Niton XRF - Handheld X-Ray.  Portable X-Ray Analyser.  Handheld Spectrometers. TruScan RM
We also offer Thermo Scientifics TruProcess (SpectralProbeTM Blender) - the next generation NIR sensor for real time blend analysis, drying, and other PAT (Process Analytical Technology) applications.  PAT for QbD.
Agilent 5890, 6890 (replaces 7673), Varian 3400, 3800, 3900 (replaces 8200 and 8400 autosamplers), Perkin Elmer, Shimadzu GC9a, GC17a, GC15a, GC14a (replaces all AOC models) plus Operational and Performance Qualification. The HT300A is now a direct plug-in replacement for the HP7673 autosampler found on HP (Agilent) 5890 and 6890 GC systems. The HT300A can be controlled from multiple software packages including Hewlett Packard Chemstation, EZ Chrom plus......
Our model HT200H offers Exceptional Headspace performance and can be installed on your existing GC.
Headspace vials available for all instruments including magnetic caps for the CTC CombiPAL.

New: HT250HD offers Liquid and Headspace sampling in 1 unit.
Tablet Dissolution, Friability, Disintegration accessories for Distek, Vankel, Erweka, Sotax, Hanson, Pharmatest including validation (IQ/OQ/PQ) services.
Is your Tablet Dissolution system conforming to USP 711?
GC MS (Gas Chromatography Mass Spectrometer) Electron Multipliers for Varian, Agilent, Perkin Elmer, Thermo, VG, PE Sciex, Kratos, Jeol, Inficon
HPLC column filing and storage bank, ReCol LC column storage and management system www.antech.ie/recol.shtml
HPLC and GC parts including seals, plungers, filters, Most consumable items available for your laboratory
Deuterium, Xenon, Mercury, HCL (AA) lamps for Waters, HP/Agilent, Shimadzu, Perkin Elmer, Varian, Thermo, Unicam

We  offer a complete range of fully reconditioned and tested systems from: HP / Agilent 1100 HPLC and GC (5890 & 6890). Waters Alliance 2690, 2695, 2487, 996, 2996, 717, 600e
We also offer: HPLC Solvent Recycler, SolventTrak, for acetonitrile shortages, isocratic solvent recycler, solvent recycler system, acetonitrile salvage
We are exclusive agents for the complete range of Copley Scientific instrumentation.Tablet Dissolution, Disintegration, Friability, Tablet Hardness testers, capsule filling, ointment preparation, powder analysis detergent testing, blister packing plus an exhaustive list of analysers for the Research, Clinical Trials, Production and Quality Control environments. All units designed to comply with Pharmacopoeia requirements. Copley Scientific has more than 50 years' experience supplying pharmaceutical test equipment to the worldwide marketplace. Test equipment for solid dosage forms, such as tablets and capsules. Combining intuitively easy-to-use operator interfaces, free from clutter and gimmicks, with easily validated pharmacopoeia compliance, this range of products is ideal for the r&d and QC laboratories. A world leader in test equipment for inhalers (MDIs and OPIs)blenders, waring blenders (liquids and suspensions), multiblend blenders (powders and granules), turbula shaker blender (powders and granules), stomacher laboratory blender (liquid, powders and suspensions), blister packers, OMAR, minipack blister sealing machine (using pre-formed blisters), twinpack semi-automatic blister packer (using PVC/Alu pre-cut sheet), universal blister packer (for both thermo and cold forming operations), de-blistering machines, calipers and gauges, digital calipers for measuring tablets, tablet thickness gauges, capsule fillers, manual capsule filler, manual capsule inserter and filler, semi-automatic capsule inserter and filler, check weighers, sorters, colour measurement, data loggers, temptale for temperature and humidity monitoring, detergent testers, tergotometer, washing machine simulator, disintegration testers, tablet disintegration testers series, automatic disintegration testers, automated disintegration testers, DISI, DTG Series, DTG 1000, 2000, 3000, 4000, DTG 2000 IS, automated tablet disintegration tester DISI, disintegration baskets, disintegration disks, tablet dissolution testers (methods 1 and 2), tablet dissolution systems, copley dissolution tester, dissolution tester model DIS 6000, 8000, tablet, also introducing the ultimate Dissolution Tester DIS-EMC, dissolution spectrophotometer, UV/VIS, HPLC, dissolution accessories, consumables, rotating basket assemblies, paddles, vessels and vessel covers, UV resistant accessories, depth and centring gauges, baskets for the determination of the dissolution rate of suppositories, cells for transdermal studies, capsule sinkers and weights, carrying cases for rotating basket assemblies and paddles, support racks, carrying racks for vessels, aquasan anti-bacterial and algae tablets, sampling accessories, sampling probes and return inserts, PVC ribbon tubing, tablet dissolution filters, USP dissolution calibrators, USP 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Installation Qualification (IQ), Operational Qualification (OQ) and Performance Qualification (PQ) Performance Verification (PV) available for a range of HPLC, GC, Atomic Absorption, UV/Vis spectrometers (spectrophotometers)

We also stock a complete range of consumables and supplies from Titan sample filters to vials and liners to columns from leading suppliers such as GC columns, glass liners, ferrules from SGE,  eVol from SGE, eVol is the first digitally controlled analytical syringe, sample vials and filters from SUN-SRi, UV,Xenon and HCL (AA) lamps from Cathodeon and UV/Vis accessories and cuvettes from Hellma. A vast range of D2 lamps, Deuterium lamps, UV lamps, AA lamps.  And alternative long life D2 lamps, Deuterium lamps. And alternative UV lamps and AA lamps.  We also supply a budget range of sample syringe filters - classic filters.
 
HPLC column filing systems . HPLC Column Storage.  Organise your HPLC Columns with ReCol HPLC Column Filing Storage Solutions.

We supply Alternative HPLC Parts & Fittings, including Pump Spares & Tubing, Kromega range.
Contact us now for further information or please browse through our website for more details.